Conformation of the Solute-Binding Protein AdcAII Influences Zinc Uptake in Streptococcus pneumoniae

ML Župan; Z Luo; K Ganio; VG Pederick; SL Neville; E Deplazes; B Kobe; CA McDevitt

Journal article

Conformation of the Solute-Binding Protein AdcAII Influences Zinc Uptake in Streptococcus pneumoniae

ML Župan, Z Luo, K Ganio, VG Pederick, SL Neville, E Deplazes, B Kobe, CA McDevitt

Frontiers in Cellular and Infection Microbiology | FRONTIERS MEDIA SA | Published : 2021

DOI: 10.3389/fcimb.2021.729981

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Abstract

Streptococcus pneumoniae scavenges essential zinc ions from the host during colonization and infection. This is achieved by the ATP-binding cassette transporter, AdcCB, and two solute-binding proteins (SBPs), AdcA and AdcAII. It has been established that AdcAII serves a greater role during initial infection, but the molecular details of how the protein selectively acquires Zn(II) remain poorly understood. This can be attributed to the refractory nature of metal-free AdcAII to high-resolution structural determination techniques. Here, we overcome this issue by separately mutating the Zn(II)-coordinating residues and performing a combination of structural and biochemical analyses on the varian..

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University of Melbourne Researchers

Stephanie Neville Author

Christopher McDevitt Author

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Awarded by Australian Government

Funding Acknowledgements

This research was undertaken with the assistance of resources and services from the National Computational Infrastructure (NCI), which is supported by the Australian Government and by resources provided by The Pawsey Supercomputing Centre with funding from the Australian Government and the Government of Western Australia. This research was also undertaken using the LIEF HPC-GPGPU Facility hosted at the University of Melbourne. This Facility was established with the assistance of Australian Research Council (ARC) LIEF Grant LE170100200. This work was supported by the National Health and Medical Research Council (NHMRC) grants 1071659 to BK, 1122582 to CM and 1180826 to BK and CM. This work was also supported by the ARC Discovery Project Grant toCM(DP170102102). SN is an NHMRC Early Career Research Fellows (1142695), ED is a UTS Chancellor's Postdoctoral Fellow, BK is an ARC Laureate Fellow (FL180100109), and CMis an ARC Future Fellow (FT170100006).